Education

• M.Sc., University of Sao Paulo, Brazil 2008
• Ph.D., The University of Queensland, Australia, 2016
• Postdoctoral Research Fellow, University of Maryland, 2017-2018
• Postdoctoral Research Fellow, Scripps Research, 2018-2023

Awards & Honors

• Meet up-and-coming analytical scientists – Fabio Pereira Gomes, Analytical Science Advances
• Faces of Mass Spectrometry/Fabio Gomes, Journal of the American Society for Mass Spectrometry
• Travel award: American Society for Mass Spectrometry on Top-Down Proteomics
• Travel award: School of Pharmacy, The University of Queensland - Australia
• Fellowship: University of Queensland Centennial Scholarship - Australia
• Fellowship: International Postgraduate Research Scholarship - Australia
• Fellowship: Coordination for Projects, Research, and Technological Studies - Brazil
• Fellowship: National Counsel of Technological and Scientific Development - Brazil
• Fellowship: Institute of Pharmaceutical Research - Brazil

Research Interests

The significance of structure-function relationships is clearly observed in several biologically relevant problems, ranging from understanding how tumors develop drug resistance to the design of effective therapeutic agents that inhibit the actions of target proteins. For instance, most cellular functions, including those linked to health and disease, are governed by protein complexes that are often formed via non-covalent interactions of monomeric protein subunits. These individual proteins adopt various modified forms “proteoforms” as a result of numerous structural changes (e.g., posttranslational modifications and truncations). Proteoforms influence the activity, formation, and stability of functional protein complexes, and they can form numerous functional “complexoforms” from a single protein complex. Moreover, individual proteoforms can independently modulate numerous biological processes, and they can also serve as important markers of disease. Dr. Gomes’ interdisciplinary research program in structural mass spectrometry (MS)-based proteomics builds on the legacy of Professor John B. Fenn’s pioneering work on the development of electrospray ionization for the MS analysis of biological macromolecules. The research in the Gomes group is focused on developing and improving bioanalytical platforms that combine chromatography, electrophoresis, ion-mobility, and mass spectrometry to probe intact proteoforms and their complexoforms within the intracellular space. These bioanalytical platforms are applied to better understand the molecular mechanisms that control metastatic tumors, drug resistance, and how lipids bind and modulate the biological activities of important drug targets such as membrane proteins. The acquired structural knowledge is then applied to identify therapeutic targets, advance the drug discovery field and ultimately, improve public health. 

Publications

Fabio P. Gomes, Kenneth R. Durbin, Kevin Schauer, Jerome C. Nwachukwu, Robin J. Russo, Jacqline W. Njeri, Anthony J. Saviola, Daniel B. McClatchy, Jolene K. Diedrich, Patrick T. Garrett, Alexandra B. Papa, Ianis Ciolacu, Neil L. Kelleher, Kendall W. Nettles, and John R. Yates III. Native top-down proteomics reveals EGFR–ERα signaling crosstalk in breast cancer cells dissociates NUTF2 dimers to modulate ER signaling and cell growth, (Under review).

Fabio P. Gomes, Jolene K. Diedrich, Anthony J. Saviola, Erdogan Memili, Arlindo A. Moura, John R. Yates, III. EThcD and 213 nm UVPD for top-down analysis of bovine seminal plasma proteoforms on electrophoretic and chromatographic time frames, Analytical Chemistry, 2020, 92, 4, 2979-2987.

Fabio P. Gomes, Robin Park, Arabela G. Viana, Carolina F. Costa, Einko Topper, Abdullah Kaya, Erdogan Memili, John R. Yates, III, Arlindo A. Moura. Protein signatures of seminal plasma from bulls with contrasting frozen-thawed sperm viability, Scientific Reports, 2020;10(1):14661.

Fabio P. Gomes and John R. Yates, III. Recent trends of capillary electrophoresis-mass spectrometry in proteomics research, Mass Spectrometry Reviews, 2019;38(6):445-460.

Fabio Gomes, Betsegaw Lemma, Dulith Abeykoon, Dapeng Chen, Yan Wang, David Fushman and Catherine Fenselau. Top-down analysis of novel synthetic branched proteins. Journal of Mass Spectrometry, 2019, 54(1):19-25.

Dapeng Chen, Lucia Geis-Asteggiante, Fabio P. Gomes, Suzanne Ostrand-Rosenberg, Catherine Fenselau. Top-down proteomic characterization of truncated proteoforms, Journal of Proteome Research, 2019;18(11):4013-4019.

Dapeng Chen#, Fabio Gomes#, Dulith Abeykoon, Betsegaw Lemma, Yan Wang, David Fushman and Catherine Fenselau. Top-down analysis of branched proteins using mass spectrometry, Analytical Chemistry, 90 (6); 2018; 4032–4038. #The first two authors contributed equally to this work.

Nan Xie, Fabio P. Gomes, Vandana Deora, Kye Gregory, Tharindu Vithanage, Zeyad D. Nassar, Peter J. Cabot, David Sturgess, Paul N. Shaw and Marie-Odile Parat. Activation of μ-opioid receptor and Toll-like receptor 4 by plasma from morphine-treated mice, Brain, Behavior, and Immunity, 61; 2017; 244-258.

Gomes F.P., Shaw P.N. and Hewavitharana A.K. Determination of four sulfated vitamin D compounds in human biological fluids by liquid chromatography-tandem mass spectrometry, J. Chromatogr. B, 1009-1010: 2016; 80–86.

Gomes F.P., Shaw P.N., Whitfield K., Koorts P., McConachy H. and Hewavitharana A.K. Effect of pasteurization on the concentrations of vitamin D compounds in donor breast milk, International Journal of Food Sciences and Nutrition, 67:1; 2016; 16-19.
15) Kassim N.S.A., Gomes F.P., Shaw P.N., and Hewavitharana, A.K. Simultaneous quantitative analysis of nine vitamin D compounds in human blood using LC-MS/MS, Bionalysis, 8:5; 2016; 397 - 411.

Hewavitharana A.K., Gomes F.P. (2016) Cholecalciferol: Properties and Determination. In: Caballero B., Finglas, P., and Toldrá, F., (eds.), The Encyclopedia of Food and Health, vol. 2., pp. 42-46. Oxford: Academic Press.

Gomes F.P., Shaw P.N., Whitfield K., Hewavitharana A.K. Simultaneous quantitative analysis of eight vitamin D analogues in milk using liquid chromatography–tandem mass spectrometry. Analytica Chimica Acta, 891: 2015; 211-220.

Gomes F.P., Shaw P.N., Whitfield K., Koorts P., Hewavitharana A.K. Recent trends in the determination of vitamin D. Bioanalysis. 5(24); 2013; 3063-78.

Gomes F.P.* and García P.L. Development and validation of a simple and sensitive high performance liquid-chromatographic method for the simultaneous determination of anastrozole, bicalutamide, tamoxifen, and their synthetic impurities, Talanta, 101: 2012; 495-503. *Corresponding author.

P. L. Garcia, F. P. Gomes, M. I. R. M. Santoro, E. R. M. Kedor-Hackmann, J. L. V. Quero, A. N. Montón, G. C. Montoya, M. A. Cabrera. Determination of vecuronium bromide in pharmaceuticals: development, validation, and comparative study of an HPLC and CZE analytical methods, Chromatographia, 73(7-8): 2011; 799-805.

Garcia P.L., Buffoni E., Gomes F.P and Quero J.L.V. (2011) Analytical Method Validation. In: Akyar I., (ed.), Wide Spectra of Quality Control. P. 3-20. Rijeka: InTech.

F. P. Gomes, P. L. Garcia, J. M. P. Alves, A. K. Singh, E. R. M. Kedor-Hackmann, M. I. R. M. Santoro. Simple and sensitive stability-indicating RP-HPLC methods for the quantitative determination of pravastatin sodium, Fluvastatin sodium, atorvastatin calcium and rosuvastatin calcium in pharmaceuticals, Analytical Letters, 42(12): 2009; 1784-1804.

F. P. Gomes, P. L. Garcia, J. M. P. Alves, A. K. Singh, E. R. M. Kedor-Hackmann, M. I. R. M. Santoro. Stability-indicating high-performance liquid chromatographic and UV-derivative spectrophotometric methods for determination of simvastatin in tablets, Latin American Journal of Pharmacy, 28(2): 2009; 261-269.

M. I. R. M. Santoro, C. Tsubone, F. P. Gomes, E. R. M. Kedor-Hackmann, P. L. Garcia. Development and validation of high-performance liquid chromatographic and UV-derivative spectrophotometric methods for the determination of sotalol hydrochloride in tablets, Analytical Letters, 41(11): 2008; 2044-2057.

P. L. Garcia, F. P. Gomes, M. I. R. M. Santoro, E. R. M. Kedor-Hackmann. Validation of an HPLC analytical method for determination of pancuronium bromide in pharmaceutical injections, Analytical Letters, 41(10): 2008; 1895–1908.

A. K. Singh, P. L. Garcia, F. P. Gomes, H. M. Yano, M. T. Auricchio, E. R. M. Kedor-Hackmann, M. I. R. M. Santoro. Development and validation of sensitive methods for determination of sibutramine hydrochloride monohydrate and direct enantiomeric separation on a protein-based chiral stationary phase, Journal of AOAC International, 91(3): 2008; 572-579.

C. Tsubone, P. L. Garcia, F. P. Gomes, E. R. M. Kedor-Hackmann, M. I. R. M. Santoro. Quantitative determination of nadolol in tablets by high-performance liquid chromatography and UV-derivative spectrophotometry, Analytical Letters, 41(3): 2008; 424-436.

A. K. Singh, P. L. Garcia, F. P. Gomes, E. R. M. Kedor-Hackman, M.R.I.M. Santoro. Comparative study on two rapid and sensitive methods for quantitative determination of tenoxican, Brazilian Journal of Pharmaceutical Science, 43(4): 2007; 615-622.

H. A. Stefani, R. Cella, F. A. Dorr, C. M. P. Pereira, F. P. Gomes, G. Zeni. Ultrasound-assisted synthesis of functionalized arylacetylenes, Tetrahedron Letters, 46(12): 2005; 2001-2003.